Alternatively, a supplier may choose to take the opposite tack and focus on a sample type that may be used less frequently by researchers in general, but employed extensively by customers in certain niche markets. For example, a supplier may choose to emphasise their expertise in purification kits for the most frequently used type of sample, and place less emphasis on those products optimised for a specific sample type. The balance of the market is fragmented among several different sample types such diversity associated with sample types can provide an opportunity for supplier differentiation, a key to success in this crowded marketplace. It may be acceptable to have a lower overall RNA yield to decrease the amount of DNA present.вЂќ Multiple sample types provide opportunity for differentiationДЄlmost half of all the samples from which RNA is purified are either established cell lines or animal tissue. As techniques become more sensitive (such as real-time PCR), it will become easier to detect such contamination and more importantly, that it is absent. As one respondent explained, purification products вЂњhave become much better recently however there is still a problem with DNA contamination. Both of these PCR applications are very sensitive and require extremely pure RNA (ie, RNA with no protein or DNA contamination), thus presenting a challenge for suppliers to create products that will satisfy this demand for increased purity. Respondents most commonly use their purified RNA for RT-PCR, qPCR and cDNA synthesis.

Suppliers may choose to differentiate themselves by expanding their product lines to provide purification products specific for these niche types of RNA. While total RNA and poly(A) mRNA are the types of RNA most commonly purified, several other types of RNA are also purified, including viral RNA, micro RNA, ribosomal RNA and small RNA. Suppliers offer a multitude of products designed for total RNA purification, and many offer products for purifying poly(A) mRNA from total RNA or directly from cells or tissue. RNA purification extends into niche marketsДЄlmost all respondents who purify RNA currently purify total RNA, followed by 45% who purify poly(A) mRNA. In summary, this report will help suppliers of RNA purification kits and reagents assess opportunities to expand their reach into additional markets. An appraisal of product attribute preferences coupled with supplier satisfaction and loyalty analysis provides insight into market dynamics.

This report provides life science suppliers with a comprehensive assessment of RNA purification practices, with a strong emphasis on what influences a researcher to utilise commercial offerings and/or homebrew techniques. The RNA Purification Market: Evaluating Competitive Dynamics & Establishing Benchmarks, a report released in May by market research firm BioInformatics, LLC (Arlington, VA), surveyed 813 scientists who currently perform RNA purification. Finally, a product portfolio must be built with a look towards future growth вЂ“ including growth from increased throughput of current end-users, new entrants to the field of RNA purification and finally, conversion of users of homebrew methods. The optimal product portfolio accounts for not only researchersвЂ™ current utilisation patterns, but also the coverage and entrenchment of competitorsвЂ™ products as well. Suppliers face the strategic imperative of ascertaining the appropriate product mix between breadth of product line and depth of coverage across varied sample types. Furthermore, researchers often develop in-house or вЂhomebrewвЂ™ purification methods because there is not a suitable commercial offering to fit their particular experimental requirements. On one hand, the need to quickly and consistently obtain pure RNA generates a demand for the convenience and quality results of commercial offerings on the other hand, the diversity of research needs fragments the potential customer base. RNA purification is an area of particular salience for life science suppliers, as the successful extraction of RNA poses not only technical challenges to researchers, but also commercial challenges of a heterogeneous marketplace due to variation in end usersвЂ™ sample sources, level of throughput and downstream applications. Purified RNA is crucial for use in a range of downstream applications performed by life scientists, most notably reverse transcription PCR (RT-PCR), quantitative real-time PCR (qPCR) and cDNA synthesis.

Purify app archive